Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer Protein

Name: Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer Protein
Brand: Puweituo Bio
SKU: EGFRL858R-HB002

Catalog numberEGFRL858R-HB002

HumanHEK293Molecule · ErbBMolecular weight · 50.40 kDa

Product introduction

Recombinant Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and KITDFGRAK peptide (accession: AAV53343.1(HLA-A*11:01) & P61769(B2M) &KITDFGRAK).

Product details

Molecular aliases: ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
Protein length: Gly25-Thr305
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 50.40 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for HLA-A*11:01&B2M&EGFR TCR with the EC50 of 3.7ng/ml determined by ELISA (QC Test).

Background

The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.

References

1.(1) Seshacharyulu P, Ponnusamy M P, Haridas D, et al. Targeting the EGFR signaling pathway in cancer therapy[J]. Expert Opinion on Therapeutic Targets, 2012.

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Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer Protein

Catalog numberEGFRL858R-HB002

HumanHEK293Molecule · ErbBMolecular weight · 50.40 kDa

Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human HLA-A*11:01&B2M&EGFR L858R (KITDFGRAK) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References