Biotinylated Human DDR1 Protein

Name: Biotinylated Human DDR1 Protein
Brand: Puweituo Bio
SKU: DDR1-HB001

Catalog numberDDR1-HB001

HumanHEK293Molecule · HGK2Molecular weight · 46.93 kDa

Product introduction

Recombinant Biotinylated Human DDR1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Asp21-Ala417 (UniProt accession: Q08345-1).

Product details

Molecular aliases: HGK2; MCK-10; CAK; EDDR1; NEP; NTRK4; PTK3A; RTK6; TRKE; CD167a; EC 2.7.10.1; MCK10; EC2.7.10.1
Protein length: Asp21-Ala417
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 46.93 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human DDR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human DDR1 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human DDR1, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA. (QC Test)

Background

Discoidin domain receptor1 (DDR1) is a collagen activated receptor tyrosine kinase and an attractive anti-fibrotic target. Its expression is mainly limited to epithelial cells located in several organs including skin, kidney, liver and lung. DDR1's biology is elusive, with unknown downstream activation pathways; however, it may act as a mediator of the stromal-epithelial interaction, potentially controlling the activation state of the resident quiescent fibroblasts.

References

1.Moll S, Desmoulière A, Moeller MJ, Pache JC, Badi L, Arcadu F, Richter H, Satz A, Uhles S, Cavalli A, Drawnel F, Scapozza L, Prunotto M. DDR1 role in fibrosis and its pharmacological targeting. Biochim Biophys Acta Mol Cell Res. 2019 Nov;1866(11):118474. doi: 10.1016/j.bbamcr.2019.04.004. Epub 2019 Apr 5. PMID: 30954571.

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Biotinylated Human DDR1 Protein

Catalog numberDDR1-HB001

HumanHEK293Molecule · HGK2Molecular weight · 46.93 kDa

Product introduction

Recombinant Biotinylated Human DDR1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Asp21-Ala417 (UniProt accession: Q08345-1).

Biotinylated Human DDR1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human DDR1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References