Biotinylated Human CXCR7 Nanodisc

Name: Biotinylated Human CXCR7 Nanodisc
Brand: Puweituo Bio
SKU: CXCR7-HB001

Catalog numberCXCR7-HB001

HumanHEK293Molecule · CXC-R7Molecular weight · 54.4 kDa

Product introduction

Recombinant Biotinylated Human CXCR7 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Lys362 (UniProt accession: P25106).

Product details

Molecular aliases: CXC-R7; CXCR-7; ACKR3; CMKOR1; GPR159; RDC1; RDC-1; CXCR7
Protein length: Met1-Lys362
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 54.4 kDa.
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Storage: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Negative control: CON-HM1NB

Data display

ELISA Data — Immobilized Biotinylated Human CXCR7 Nanodisc, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CXCR7 Antibody 1, hFc Tag with the EC50 of 24.2ng/ml determined by ELISA. (QC Test)

SPR Data — Biotinylated Human CXCR7 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CXCR7 Antibody 1, hFc Tag with an affinity constant of 3.22 nM as determined in SPR assay (Biacore T200).

Background

Atypical chemokine receptor 3 (ACKR3), previously known as C-X-C chemokine receptor type 7 (CXCR7), has emerged as a key player in several biologic processes, particularly during development. Its CXCL11 and CXCL12 scavenging activity and atypical signaling properties, together with a new array of other nonchemokine ligands, have established ACKR3 as a main regulator of physiologic processes at steady state and during inflammation.

References

1.Koenen J, Bachelerie F, Balabanian K, Schlecht-Louf G, Gallego C. Atypical Chemokine Receptor 3 (ACKR3): A Comprehensive Overview of its Expression and Potential Roles in the Immune System. Mol Pharmacol. 2019 Dec;96(6):809-818. doi: 10.1124/mol.118.115329. Epub 2019 Apr 30. PMID: 31040166.

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Biotinylated Human CXCR7 Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

Background

References

Biotinylated Human CXCR7 Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

Background

References