Biotinylated Human Claudin 3 Nanodisc Protein

Name: Biotinylated Human Claudin 3 Nanodisc Protein
Brand: Puweituo Bio
SKU: CLDN3-HB001

Catalog numberCLDN3-HB001

HumanHEK293Molecule · Claudin-3

Product introduction

Biotinylated Human Claudin 3 Nanodisc Protein is a full-length multi-pass membrane protein presented in a Nanodisc format for antibody discovery, binding assays and method development. Recombinant Biotinylated Human Claudin 3 Nanodisc is expressed from HEK293 with His tag at the C-terminus. It contains Met1-Val220 (accession: O15551).

Product details

Molecular aliases: Claudin-3; CPE-R 2; CPE-receptor 2; hRVP1; CLDN3; C7orf1; CPETR2; RVP1
Protein length: Met1-Val220
Expression system: HEK293
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Storage: Valid for 12 months from date of receipt when stored at -80°C. Aliquot where appropriate and minimize freeze-thaw cycles.

Background

Claudin-3 is a memember of the Claudin family, which is present in many epithelia including skin, lung, kidney and intestine. It beautifully localizes on the cell-to-cell junctions of MCF-7 cells, clearly forming a continuous belt-like structure surrounding the cells. It has often been associated with the progression and metastasis of various tumors.

References

1.Milatz S, Krug SM, Rosenthal R, Günzel D, Müller D, Schulzke JD, Amasheh S, Fromm M. Claudin-3 acts as a sealing component of the tight junction for ions of either charge and uncharged solutes. Biochim Biophys Acta. 2010 Nov;1798(11):2048-57. doi: 10.1016/j.bbamem.2010.07.014. Epub 2010 Jul 22. PMID: 20655293.

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