Biotinylated Human CDH17/Cadherin 17 Domain 5-7 Protein

Name: Biotinylated Human CDH17/Cadherin 17 Domain 5-7 Protein
Brand: Puweituo Bio
SKU: CDH17-HB002

Catalog numberCDH17-HB002

HumanHEK293Molecule · Cadherin-17Molecular weight · 38.6 kDa

Product introduction

Recombinant Biotinylated Human CDH17/Cadherin 17 Domain 5-7 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Glu450-Gly777 (UniProt accession: Q12864).

Product details

Molecular aliases: Cadherin-17; BILL-cadherin; LI-cadherin; P130; Cdh17; CDH16; HPT-1; cadherin-16; FLJ26931; MGC138218; MGC142024; HPT1
Protein length: Glu450-Gly777
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 38.6 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Human CDH17 Domain 5-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data — Immobilized Biotinylated Human CDH17 Domain 5-7, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CDH17 Domain 5-7 Antibody, hFc Tag with the EC50 of 10.7ng/ml determined by ELISA. (QC Test)

BLI Data — Loaded Anti-CDH17 Domain 5-7 Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated CDH17 Domain 5-7, His Tag with an affinity constant of 2.89 nM as determined in BLI assay.

Background

Liver-intestine cadherin (CDH17) has been known to function as a tumor stimulator and diagnostic marker for almost two decades. In vivo studies showed CDH17 knockout resulted in apoptotic PC tumor death through activating caspase-3 activity. Taken together, CDH17 functions as an oncogenic molecule critical to PC growth by regulating tumor apoptosis signaling pathways and CDH17 could be targeted to develop an anti-PC therapeutic approach.

References

1.Liu X, Huang Y, Yuan H, Qi X, Manjunath Y, Avella D, Kaifi JT, Miao Y, Li M, Jiang K, Li G. Disruption of oncogenic liver-intestine cadherin (CDH17) drives apoptotic pancreatic cancer death. Cancer Lett. 2019 Jul 10;454:204-214. doi: 10.1016/j.canlet.2019.04.022. Epub 2019 Apr 17. PMID: 31004701.

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Biotinylated Human CDH17/Cadherin 17 Domain 5-7 Protein

Catalog numberCDH17-HB002

HumanHEK293Molecule · Cadherin-17Molecular weight · 38.6 kDa

Biotinylated Human CDH17/Cadherin 17 Domain 5-7 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

BLI

BLI Data

Background

References

Biotinylated Human CDH17/Cadherin 17 Domain 5-7 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

BLI

BLI Data

Background

References