Human CEACAM-5/CD66e Protein

Name: Human CEACAM-5/CD66e Protein
Brand: Puweituo Bio
SKU: CD66E-HF001

Catalog numberCD66E-HF001

HumanHEK293Molecule · CEACAM-5Molecular weight · 97.27 kDa

Product introduction

Recombinant Human CEACAM-5/CD66e Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Lys35-Ala685 (UniProt accession: P06731).

Product details

Molecular aliases: CEACAM-5; CD66e; CEA; Meconium antigen 100; Carcinoembryonic; CEACD66e; DKFZp781M2392;CEACAM5
Protein length: Lys35-Ala685
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 97.27 kDa. Due to glycosylation, the protein migrates to 160-200 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Human CEACAM-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human CEACAM-5 is greater than 90% as determined by SEC-HPLC.

ELISA Data — Immobilized Human CEACAM-5, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CEACAM-5 Antibody, hFc Tag with the EC50 of 25.8ng/ml determined by ELISA. (QC Test)

Background

Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) was identified as a metastatic driver. CEACAM5 overproduction enriched for an epithelial gene expression pattern and facilitated tumor outgrowth at metastatic sites. Tissues from patients with metastatic breast cancer confirmed elevated levels of CEACAM5 in lung metastases relative to breast tumors, and an inverse correlation between CEACAM5 and the mesenchymal marker vimentin was demonstrated.

References

1.Li Q, Li Y, Li J, et al. FBW7 suppresses metastasis of colorectal cancer by inhibiting HIF1α/CEACAM5 functional axis. Int J Biol Sci. 2018;14(7):726-735. Published 2018 May 12. doi:10.7150/ijbs.24505

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Human CEACAM-5/CD66e Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Human CEACAM-5/CD66e Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References