Human CD52 Protein

Name: Human CD52 Protein
Brand: Puweituo Bio
SKU: CD52-HF002

Catalog numberCD52-HF002

HumanHEK293Molecule · CD52 moleculeMolecular weight · 27.6 kDa

Product introduction

Recombinant Human CD52 Protein is expressed in HEK293 cells with an mFc (IgG1) tag at the C-terminus. It contains amino acid residues Gly25-Ser36 (UniProt accession: P31358).

Product details

Molecular aliases: CD52 molecule; CD52; CDW52 antigen (CAMPATH-1 antigen); CDw52; He5; HEL-S-171mP; CAMPATH-1; CAMPATH1;CDW52 antigen (CAMPATH1 antigen); HEL-S171mP
Protein length: Gly25-Ser36
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 27.6 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human CD52 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human CD52 is greater than 95% as determined by SEC-HPLC.

Background

CD52, also known as CAMPATH-1 antigen, HE5, and gp20, is a cell surface glycoprotein that can be targeted to induce immune suppression by complement-mediated cell lysis. Mature human CD52 is a 12 amino acid peptide that is tethered to the cell surface with a GPI linkage.CD52 may play a role in carrying and orienting carbohydrate, as well as having a more specific role.

References

1.Jiang L, Yuan C M, Hubacheck J, et al. Variable CD52 Expression in Mature T Cell and NK Cell Malignancies: Implications for Alemtuzumab Therapy[J]. British Journal of Haematology, 2009, 145(2):173-179. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Human CD52 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References

Human CD52 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References