Human CD44v6 Protein

Name: Human CD44v6 Protein
Brand: Puweituo Bio
SKU: CD44V6-HF001

Catalog numberCD44V6-HF001

HumanHEK293Molecule · CD44Molecular weight · 53.57 kDa

Product introduction

Recombinant Human CD44v6 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gln21-Pro220 & Gln386-Ala427 (UniProt accession: P16070-1).

Product details

Molecular aliases: CD44; CDw44; Epican; ECMR-III; LHR; MDU2; MDU3; MIC4; PGP-I; CSPG8; HCELL; HUTCH-I; IN; MC56
Protein length: Gln21-Pro220
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 53.57 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human CD44v6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human CD44v6 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human CD44v6, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD44 Antibody, hFc Tag with the EC50 of 36.6ng/ml determined by ELISA (QC Test).

Background

CD44 is a hyaluronan binding cell surface signal transducing receptor that influences motility, cell survival and proliferation as well as the formation of tumor microenvironment. CD44 contains two variable regions encoded by variable exons. Alternative splicing, which is often deregulated in cancer, can produce various isoforms of CD44 with properties that may have different tissue specific effects and therefore even diverse effects on cancer progression

References

1.Prochazka L, Tesarik R, Turanek J. Regulation of alternative splicing of CD44 in cancer. Cell Signal. 2014 Oct;26(10):2234-9. doi: 10.1016/j.cellsig.2014.07.011. Epub 2014 Jul
2.PMID:
3.Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Human CD44v6 Protein

Catalog numberCD44V6-HF001

HumanHEK293Molecule · CD44Molecular weight · 53.57 kDa

Product introduction

Recombinant Human CD44v6 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gln21-Pro220 & Gln386-Ala427 (UniProt accession: P16070-1).

Human CD44v6 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Human CD44v6 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References