Human CD42b/GP1BA Protein

Name: Human CD42b/GP1BA Protein
Brand: Puweituo Bio
SKU: CD42B-HF001

Catalog numberCD42B-HF001

HumanHEK293Molecule · GP-Ib alphaMolecular weight · 82.47 kDa

Product introduction

Recombinant Human CD42b/GP1BA Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues His17-Leu531 (UniProt accession: P07359).

Product details

Molecular aliases: GP-Ib alpha; GPIb-alpha; GPIbA; GP1BA; BDPLT1; BDPLT3; BSS; CD42B; CD42b-alpha; DBPLT3; GP1B; VWDP; BP1BA; MGC34595
Protein length: His17-Leu531
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 82.47 kDa. Due to glycosylation, the protein migrates to 130-170 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 0.1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS, 100mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human CD42b on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human CD42b is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human CD42b, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD42b Antibody, hFc Tag with the EC50 of 21.1ng/ml determined by ELISA. (QC Test)

Background

Absence of the CD42 complex leads to the Bernard-Soulier syndrome (BSS), which is a bleeding disorder characterized by thrombocytopenia and giant platelets. So far, mutations have been found in the genes encoding CD42a, CD42b and CD42c in patients with BSS**.

References

1.Clemetson KJ,McGregor JL,James E,Dechavanne M,Luscher EF Characterization of the platelet membrane glycoprotein abnormalities in Bernard-Soulier syndrome and comparison with normal by surface-labeling techniques and high-resolution two-dimensional gel electrophoresis. J Clin Invest 1982 70:304 Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Human CD42b/GP1BA Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Human CD42b/GP1BA Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References