Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein

Name: Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein
Brand: Puweituo Bio
SKU: CD3ED-HB001

Catalog numberCD3ED-HB001

HumanHEK293Molecule · CD3Molecular weight · 40 kDa

Product introduction

Recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed in HEK293 cells with an hFc (IgG1) tag and Avi tag at the C-terminus of CD3E and hFc (IgG1) tag at the C-terminus of CD3D. It contains amino acid residues Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D) (accession: P07766(CD3E) & P04234(CD3D)).

Product details

Molecular aliases: CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon
Protein length: Asp23-Asp126
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 40 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 48-60 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human CD3E&CD3D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human CD3E&CD3D, hFc Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 42.1ng/ml determined by ELISA (QC Test).

SPR Data — Anti-CD3 Antibody, hFc Tag immobilized on CM5 Chip can bind Biotinylated Human CD3E&CD3D, hFc-Avi tag with an affinity constant of 0.17 µM as determined in SPR assay (Biacore T200).

Background

T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D, are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

References

1.Penaranda C, Tang Q, Bluestone J A. Anti-CD3 Therapy Promotes Tolerance by Selectively Depleting Pathogenic Cells while Preserving Regulatory T Cells[J]. The Journal of Immunology, 2011, 187(4):2015-2022.

加载中…

Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein

Catalog numberCD3ED-HB001

HumanHEK293Molecule · CD3Molecular weight · 40 kDa

Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

Background

References

Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

Background

References