Biotinylated Cynomolgus Siglec-15/CD33L3 Protein

Name: Biotinylated Cynomolgus Siglec-15/CD33L3 Protein
Brand: Puweituo Bio
SKU: CD33L3-CB001

Catalog numberCD33L3-CB001

HEK293Molecule · CD33 molecule-like 3Molecular weight · 29.2 kDa

Product introduction

Recombinant Biotinylated Cynomolgus Siglec-15/CD33L3 Protein is expressed in HEK293 cells with a His and Avi tag at the C-terminus. It contains amino acid residues Met60-Thr322 (accession: XP_005586866.2).

Product details

Molecular aliases: CD33 molecule-like 3; CD33L3; HsT1361; Siglec15; CD33 antigen-like 3; SIGLEC-15
Protein length: Met60-Thr322
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 29.2 kDa. Due to glycosylation, the protein migratesto 33-43 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22μm filtered solution in 20mM PB, 500mM NaCl, 0.1M L-arginine (pH 6.0).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Cynomolgus Siglec-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Cynomolgus Siglec-15 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Cynomolgus Siglec-15, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).

Background

Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.

References

1.Jun Wang,et al. Siglec-15 as an immune suppressor and potential target for normalization cancer immunotherapy. Nature Medicine
2.

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Biotinylated Cynomolgus Siglec-15/CD33L3 Protein

Catalog numberCD33L3-CB001

HEK293Molecule · CD33 molecule-like 3Molecular weight · 29.2 kDa

Biotinylated Cynomolgus Siglec-15/CD33L3 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Cynomolgus Siglec-15/CD33L3 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References