- Molecular aliases
- CD33 molecule; CD33; FLJ00391; gp67; Siglec3; Siglec-3; p67
- Protein length
- Asp18-His259
- Expression system
- HEK293
- Molecular weight
- The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Formulation
- Lyophilized from 0.22 μm filtered solution in 20 mM MES, 500 mM NaCl, 200 mM L-Arginine (pH 5.0). Normally 8% mannitol is added as protectant before lyophilization.
- State
- Lyophilized
- Storage
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
