Biotinylated Human IL-17R alpha/CD217 Protein

Name: Biotinylated Human IL-17R alpha/CD217 Protein
Brand: Puweituo Bio
SKU: CD217-HB001

Catalog numberCD217-HB001

HumanHEK293Molecule · CD217Molecular weight · 36.4 kDa

Product introduction

Recombinant Biotinylated Human IL-17R alpha/CD217 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Leu33-Trp320 (UniProt accession: Q96F46-1).

Product details

Molecular aliases: CD217; CDw217; IL-17RA; IL17R; CANDF5; IL-17R; IL-17R-alpha; IL17Rα;IL17RA; IL17R-alpha;IL17R alpha
Protein length: Leu33-Trp320
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 36.4 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human IL-17R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human IL-17R alpha is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human IL-17A, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, His Tag with the EC50 of 29.9ng/ml determined by ELISA (QC Test).

Background

Interleukin 17 (also known as CTLA‑8) is a T cell‑expressed pleotropic cytokine. IL‑17 binds to IL‑17 receptor (IL‑17 R) which shares no homology with any known family of receptors. While the expression of IL‑17 is restricted to activated T cells, the IL‑17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22.

References

1.Gaffen S L. An overview of IL-17 function and signaling[J]. Cytokine, 2008, 43(3):0-407.

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Biotinylated Human IL-17R alpha/CD217 Protein

Catalog numberCD217-HB001

HumanHEK293Molecule · CD217Molecular weight · 36.4 kDa

Biotinylated Human IL-17R alpha/CD217 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human IL-17R alpha/CD217 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References