Biotinylated Cynomolgus CD161 Protein

Name: Biotinylated Cynomolgus CD161 Protein
Brand: Puweituo Bio
SKU: CD161-CB001

Catalog numberCD161-CB001

HEK293Molecule · KLRB1Molecular weight · 21.5 kDa

Product introduction

Recombinant Biotinylated Cynomolgus CD161 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln67-Leu227 (accession: XP_005570142.1).

Product details

Molecular aliases: KLRB1; CLEC5B; NKRP1A; NKR-P1A; HNKR-P1a; CD161; Ly59; NKR; NKRP1; NKR-P1; NKRP1ANKR
Protein length: Gln67-Leu227
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 21.5 kDa. Due to glycosylation, the protein migrates to 37-42 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Cynomolgus CD161 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Cynomolgus CD161 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Cynomolgus CD161, His Tag at 0.5μg/ml (100μl/Well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 21.2ng/ml determined by ELISA.

Background

CD161 (NKRP1) is a lectin-like receptor present on NK cells and rare T-cell subsets. We have observed CD161 expression in some cases of T-cell prolymphocytic leukemia (T-PLL) and found it to be useful in follow-up and detection of disease after treatment.

References

1.Gilles SR, Yohe SL, Linden MA, Dolan M, Hirsch B, Grzywacz B. CD161 Is Expressed in a Subset of T-Cell Prolymphocytic Leukemia Cases and Is Useful for Disease Follow-up. Am J Clin Pathol. 2019 Sep 9;152(4):471-478. doi: 10.1093/ajcp/aqz060. PMID: 31305900.

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Biotinylated Cynomolgus CD161 Protein

Catalog numberCD161-CB001

HEK293Molecule · KLRB1Molecular weight · 21.5 kDa

Product introduction

Recombinant Biotinylated Cynomolgus CD161 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln67-Leu227 (accession: XP_005570142.1).

Biotinylated Cynomolgus CD161 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Cynomolgus CD161 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References