Human IL-3 R Beta/CD131 Protein

Name: Human IL-3 R Beta/CD131 Protein
Brand: Puweituo Bio
SKU: CD131-HF001

Catalog numberCD131-HF001

HumanHEK293Molecule · CDw131Molecular weight · 74.63 kDa

Product introduction

Recombinant Human IL-3 R Beta/CD131 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Thr17-Trp443 (UniProt accession: P32927-1).

Product details

Molecular aliases: CDw131; CD131; CSF2RB; IL3RB; IL5RB; AIC2A; Csfgmrb
Protein length: Thr17-Trp443
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 74.63 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Human IL-3 R Beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human IL-3 R Beta is greater than 90% as determined by SEC-HPLC.

SPR Data — Human IL-3 R Beta, hFc Tag captured on CM5 Chip via Protein A can bind Human GM-CSF, No Tag with an affinity constant of 6.78 μM as determined in SPR assay (Biacore T200) (QC Test).

Background

Interleukin-3 receptor (IL-3R) is a heterodimer that comprises an IL-3 specific alpha chain (IL-3R alpha) and a common beta chain (beta C) that is shared with the receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-5. The beta c subunit does not bind any cytokine by itself but is involved in the formation of high-affinity functional receptors for IL-5 and GM-CSF. These receptors belong to the cytokine receptor superfamily, but they are structurally and functionally more related to each other and thus make up a distinct subfamily.

References

1.Stomski FC, Sun Q, Bagley CJ, Woodcock J, Goodall G, Andrews RK, Berndt MC, Lopez AF. Human interleukin-3 (IL-3) induces disulfide-linked IL-3 receptor alpha- and beta-chain heterodimerization, which is required for receptor activation but not high-affinity binding. Mol Cell Biol. 1996 Jun;16(6):3035-46. doi: 10.1128/MCB.16.6.3035. PMID: 8649415; PMCID: PMC231298.
2.Hara T, Miyajima A. Function and signal transduction mediated by the interleukin 3 receptor system in hematopoiesis. Stem Cells. 1996 Nov;14(6):605-18. doi: 10.1002/stem.140605. PMID: 8948019.

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Human IL-3 R Beta/CD131 Protein

Catalog numberCD131-HF001

HumanHEK293Molecule · CDw131Molecular weight · 74.63 kDa

Product introduction

Recombinant Human IL-3 R Beta/CD131 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Thr17-Trp443 (UniProt accession: P32927-1).

Human IL-3 R Beta/CD131 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

Background

References

Human IL-3 R Beta/CD131 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

Background

References