Human IL-4 R alpha/CD124 Protein

Name: Human IL-4 R alpha/CD124 Protein
Brand: Puweituo Bio
SKU: CD124-HF001

Catalog numberCD124-HF001

HumanHEK293Molecule · CD124Molecular weight · 50.7 kDa

Product introduction

Recombinant Human IL-4 R alpha/CD124 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Met26-His232 (UniProt accession: P24394-1).

Product details

Molecular aliases: CD124; IL-4R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124;IL4R alpha; IL4R subunit alpha; IL4Ra
Protein length: Met26-His232
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 50.7 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS, 8% trehalose (pH 7.4).
State: Lyophilized
Storage: -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human IL-4 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human IL-4 R alpha is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human IL-4, No Tag (Cat. IL4-HM001) at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA. (QC Test)

BLI Data — Loaded Human IL-4 R alpha, hFc Tag on ProA-Biosensor can bind Human IL-4, No Tag (Cat. IL4-HM001) with an affinity constant of 0.54 nM as determined in BLI assay.

Background

Interleukin 4 Receptor alpha (IL-4 Ra), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells.

References

1.Ghoreschi K, Thomas P, Breit S, et al. Interleukin-4 therapy of psoriasis induces Th2 responses and improves human autoimmune disease[J]. Nature Medicine, 2002, 9(1):40-46. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

加载中…

Human IL-4 R alpha/CD124 Protein

Catalog numberCD124-HF001

HumanHEK293Molecule · CD124Molecular weight · 50.7 kDa

Product introduction

Recombinant Human IL-4 R alpha/CD124 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Met26-His232 (UniProt accession: P24394-1).

Human IL-4 R alpha/CD124 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

BLI

BLI Data

Background

References

Human IL-4 R alpha/CD124 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

BLI

BLI Data

Background

References