Human TNFR2/CD120b/TNFRSF1B Protein

Name: Human TNFR2/CD120b/TNFRSF1B Protein
Brand: Puweituo Bio
SKU: CD120B-HF002

Catalog numberCD120B-HF002

HumanHEK293Molecule · CD120bMolecular weight · 51.5 kDa

Product introduction

Recombinant Human TNF R2/CD120b/TNFRSF1B Protein is expressed in HEK293 cells with an mFc (IgG1) tag at the C-terminus. It contains amino acid residues Leu23-Asp257 (UniProt accession: P20333).

Product details

Molecular aliases: CD120b; Etanercept; p75TBPII; p75TNFR; TNF RII; TNF-R2; TNF-R75; TNFR80; TNFRSF1B; TNFR2; TBPII; TNFBR; TNFR1B
Protein length: Leu23-Asp257
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 51.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human TNFR2 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human TNFR2 is greater than 90% as determined by SEC-HPLC.

ELISA Data — Immobilized Human TNF alpha, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 45.8ng/ml determined by ELISA (QC Test).

SPR Data — Human TNF alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human TNFR2, mFc Tag with an affinity constant of 1.62 nM as determined in SPR assay (Biacore T200).

Background

Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha.

References

1.Vanamee, éva S, Faustman D L. TNFR2: A Novel Target for Cancer Immunotherapy[J]. Trends in Molecular Medicine, 2017:S1471491417301697. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Human TNFR2/CD120b/TNFRSF1B Protein

Catalog numberCD120B-HF002

HumanHEK293Molecule · CD120bMolecular weight · 51.5 kDa

Product introduction

Recombinant Human TNF R2/CD120b/TNFRSF1B Protein is expressed in HEK293 cells with an mFc (IgG1) tag at the C-terminus. It contains amino acid residues Leu23-Asp257 (UniProt accession: P20333).

Human TNFR2/CD120b/TNFRSF1B Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

Background

References

Human TNFR2/CD120b/TNFRSF1B Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

Background

References