Biotinylated Human Nectin-3/CD113 Protein

Name: Biotinylated Human Nectin-3/CD113 Protein
Brand: Puweituo Bio
SKU: CD113-HB001

Catalog numberCD113-HB001

HumanHEK293Molecule · CD113Molecular weight · 40.6 kDa

Product introduction

Recombinant Biotinylated Human Nectin-3/CD113 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly58-Asp400 (UniProt accession: Q9NQS3-1).

Product details

Molecular aliases: CD113; CDw113; FLJ90624; PPR3; PVRL3; PVRR3; PRR3
Protein length: Gly58-Asp400
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 40.6 kDa. Due to glycosylation, the protein migrates to 68-82 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in 20mM PB, 250mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human Nectin-3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human Nectin-3 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human Nectin-3, His-Avi tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test).

SPR Data — Biotinylated Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.13 μM as determined in SPR assay (Biacore T200).

Background

The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein.

References

1.Inagaki, M. Roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development[J]. Development, 132(7):1525-1537.

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Biotinylated Human Nectin-3/CD113 Protein

Catalog numberCD113-HB001

HumanHEK293Molecule · CD113Molecular weight · 40.6 kDa

Biotinylated Human Nectin-3/CD113 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Background

References

Biotinylated Human Nectin-3/CD113 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Background

References