Biotinylated Human CCR9 Nanodisc

Name: Biotinylated Human CCR9 Nanodisc
Brand: Puweituo Bio
SKU: CCR9-HB001

Catalog numberCCR9-HB001

HumanHEK293Molecule · C-C CKR-9Molecular weight · 54.9 kDa

Product introduction

Recombinant Biotinylated Human CCR9 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Leu369 (UniProt accession: P51686-1).

Product details

Molecular aliases: C-C CKR-9; CC-CKR-9; CCR-9; GPR-9-6; CDw199; GPR28; C-C CKR9; CC-CKR9; CCR9; GPR9-6
Protein length: Met1-Leu369
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 54.9 kDa.
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Storage: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Negative control: CON-HM1NB

Data display

ELISA Data — Immobilized Biotinylated Human CCR9 Nanodisc, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate(5μg/ml). Dose response curve for Anti-CCR9 Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA. (QC Test)

SPR Data — Biotinylated Human CCR9 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CCR9 Antibody, mFc Tag with an affinity constant of 5.90 nM as determined in SPR assay (Biacore T200).

Background

Chemokine receptor CCR9 is a G protein-coupled receptor and expressed on several types of immune cells, including dendritic cells (DCs), CD4+ T cells, and B cells. CCR9 drives the migration of immune cells to gradients of its cognate ligand CCL25 to promote tumor proliferation and metastasis.

References

1.Pathak M, Lal G. The Regulatory Function of CCR9+ Dendritic Cells in Inflammation and Autoimmunity. Front Immunol. 2020 Oct 2;11:536326. doi: 10.3389/fimmu.2020.536326. PMID: 33123124; PMCID: PMC7566413.

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Biotinylated Human CCR9 Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

SPR

SPR Data

Background

References

Biotinylated Human CCR9 Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

SPR

SPR Data

Background

References