PE-Labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein

Name: PE-Labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein
Brand: Puweituo Bio
SKU: AFP-HP010

Catalog numberAFP-HP010

HumanHEK293Molecule · Alpha-fetoprotein

Product introduction

Recombinant PE-Labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. PE-labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FMNKFIYEI peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) & FMNKFIYEI).

Product details

Molecular aliases: Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-feto; AFP; HPAFP; AFPD; FETA; MHC
Protein length: Gly25-Thr305
Expression system: HEK293
Formulation: Supplied as 0.22μm filtered solution in PBS, 0.2% BSA (pH 7.4).
Storage: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

ELISA Data — Immobilized PE-Labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&AFP (FMNKFIYEI) Antibody, hFc Tag with the EC50 of 36.8ng/ml determined by ELISA.

Background

Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response.

References

1.(1) Liu H, Xu Y, Xiang J, Long L, Green S, Yang Z, Zimdahl B, Lu J, Cheng N, Horan LH, Liu B, Yan S, Wang P, Diaz J, Jin L, Nakano Y, Morales JF, Zhang P, Liu LX, Staley BK, Priceman SJ, Brown CE, Forman SJ, Chan VW, Liu C. Targeting Alpha-Fetoprotein (AFP)-MHC Complex with CAR T-Cell Therapy for Liver Cancer. Clin Cancer Res. 2017 Jan 15;23(2):478-488. doi: 10.1158/1078-0432.CCR-16-1203. Epub 2016 Aug 17. PMID: 27535982.

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PE-Labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein

Catalog numberAFP-HP010

HumanHEK293Molecule · Alpha-fetoprotein

PE-Labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein

Product introduction

Product details

Data display

ELISA

ELISA Data

Background

References

PE-Labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein

Product introduction

Product details

Data display

ELISA

ELISA Data

Background

References