Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein, Ultra Low Endotoxin

Name: Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein, Ultra Low Endotoxin
Brand: Puweituo Bio
SKU: AFP-HP004

Catalog numberAFP-HP004

HumanHEK293Molecule · Alpha-fetoproteinMolecular weight · 258 kDa

Product introduction

Recombinant Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and FMNKFIYEI peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) & FMNKFIYEI).

Product details

Molecular aliases: Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-feto; AFP; HPAFP; AFPD; FETA; MHC
Protein length: Gly25-Thr305
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 0.01 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&AFP (FMNKFIYEI) Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA (QC Test).

SPR Data — Human HLA-A*02:01&B2M&AFP (FMNKFIEl) Tetramer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR with an affinity constant of 0.111 μM as determined in SPR assay (Biacore T200).

Background

Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response.

References

1.(1) Liu H, Xu Y, Xiang J, Long L, Green S, Yang Z, Zimdahl B, Lu J, Cheng N, Horan LH, Liu B, Yan S, Wang P, Diaz J, Jin L, Nakano Y, Morales JF, Zhang P, Liu LX, Staley BK, Priceman SJ, Brown CE, Forman SJ, Chan VW, Liu C. Targeting Alpha-Fetoprotein (AFP)-MHC Complex with CAR T-Cell Therapy for Liver Cancer. Clin Cancer Res. 2017 Jan 15;23(2):478-488. doi: 10.1158/1078-0432.CCR-16-1203. Epub 2016 Aug 17. PMID: 27535982.

加载中…

Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein, Ultra Low Endotoxin

Catalog numberAFP-HP004

HumanHEK293Molecule · Alpha-fetoproteinMolecular weight · 258 kDa

Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein, Ultra Low Endotoxin

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

Background

References

Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein, Ultra Low Endotoxin

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

Background

References