分子别名 (Synonym)
PAI; PAI-1; Serpin E1; SERPINE1; PAI1; PLANH1
来源/表达系统 (Source)
Recombinant Human PAI-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val24-Pro402 [Accession | P05121-1].
分子量
The protein has a predicted MW of 43.86 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
内毒素 (Endotoxin)
Less than 1 EU per μg by the LAL method.
纯度 (Purity)
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
制剂 (Formulation)
Lyophilized from 0.22 μm filtered solution in 50mM NaAC, 0.1M NaCl, 100mM L-arginine (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
重构方法 (Reconstitution)
Dissolve the lyophilized protein in 50mM NaAC (pH 5.5). Please refer to the Certificate of Analysis for detailed instructions.
存储 (Storage)
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
