分子别名 (Synonym)
BTN3A1&BTN2A1; BTN2A1&BTN3A1;
来源/表达系统 (Source)
Recombinant Human BTN2A1&BTN3A1 complex Protein is expressed from HEK293 with His tag at the C-terminus of BTN2A1 and Flag tag at the C-terminus of BTN3A1.It contains Gln29-Ala248 (BTN2A1) acidic tail and Gln30-Gly254 (BTN3A1) basic tail [Accession | Q7KYR7-1(BTN2A1)&O00481-1(BTN3A1)].
分子量
The protein has a predicted MW of 30.74 kDa (BTN2A1) and 30.25 kDa (BTN3A1). Due to glycosylation, the protein migrates to 53-63 kDa and 33-38 kDa based on Bis-Tris PAGE result.
内毒素 (Endotoxin)
Less than 1 EU per μg by the LAL method.
纯度 (Purity)
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
制剂 (Formulation)
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法 (Reconstitution)
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
存储 (Storage)
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
