分子别名 (Synonym)
S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
来源/表达系统 (Source)
Recombinant SARS-COV-2 Spike S1(Deltacron) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln14-Arg683(T19R, A27S, T95I, G142D, EFR156G, NL211I, INS214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H). Alanine substitutions (R683A) are introduced to stabilize the trimeric prefusion state of SARS-COV-2 Spike S1(Deltacron) Protein and abolish the furin cleavage site, respectively [Accession | QHD43416.1].
分子量
The protein has a predicted MW of 73.94 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result.
内毒素 (Endotoxin)
Less than 1 EU per μg by the LAL method.
纯度 (Purity)
> 95% as determined by Bis-Tris PAGE
制剂 (Formulation)
Supplied as 0.22μm filtered solution in PBS (pH 7.4).
存储 (Storage)
Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
