SARS-COV-2 Spike S1 (N501Y) Protein

Name: SARS-COV-2 Spike S1 (N501Y) Protein
Brand: 普维拓生物
SKU: PR486101

货号PR486101

核心优势

高纯度

表达宿主 / 体系: HEK293
种属 (Species): —
标签: —
纯度 (Purity): > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
活性 / 数据: 详询活性数据

代表性质控图

技术参数

分子别名 (Synonym)

S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1

来源/表达系统 (Source)

Recombinant SARS-COV-2 Spike S1 (N501Y) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val16-Arg685 [Accession | YP_009724390.1].

分子量

The protein has a predicted MW of 76 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result.

内毒素 (Endotoxin)

Less than 1 EU per μg by the LAL method.

纯度 (Purity)

> 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC

制剂 (Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法 (Reconstitution)

Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

存储 (Storage)

-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

文献引用

(1)Kemp SA, Collier DA, Datir R, Ferreira I, Gayed S, Jahun A, Hosmillo M, Rees-Spear C, Mlcochova P, Lumb IU, Roberts DJ, Chandra A, Temperton N, Sharrocks K, Blane E, Briggs J, van Gils MJ, Smith K, Bradley JR, Smith C, Doffinger R, Ceron-Gutierrez L, Barcenas-Morales G, Pollock DD, Goldstein RA, Smielewska A, Skittrall JP, Gouliouris T, Goodfellow IG, Gkrania-Klotsas E, Illingworth C, McCoy LE, Gupta RK. Neutralising antibodies in Spike mediated SARS-CoV-2 adaptation. medRxiv [Preprint]. 2020 Dec 29:2020.12.05.20241927. doi: 10.1101/2020.12.05.20241927 PMID: 33398302; PMCID: PMC7781345.

背景介绍

A new variant of SARS-CoV-2 is spreading in the UK and is rapidly becoming a global threat.It is characterised by multiple mutations in the spike protein. Among them, N501Y is of major concern because it involves one of the six key amino acid residues determining a tight interaction of the SARS-CoV-2 receptor-binding domain (RBD) with its cellular receptor angiotensin-converting enzyme 2 (ACE2).

FOLR1-PH1101

人FOLR1蛋白

SARS-COV-2 Spike S1 (N501Y) Protein

技术参数

文献引用

背景介绍

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SARS-COV-2 Spike S1 (N501Y) Protein

技术参数

文献引用

背景介绍

相关产品

定制服务与主题入口